reproff2 reprocell rchemd006 Search Results


reproff2  (ReproCELL)
90
ReproCELL reproff2
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Reproff2, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/Primate+ES+cell+medium/pmc05278902-775-4-5
Average 90 stars, based on 1 article reviews
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stemfitâ basic02 - stem cell culture media  (AMS Biotechnology)
96
AMS Biotechnology stemfitâ basic02 - stem cell culture media
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Stemfitâ Basic02 Stem Cell Culture Media, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/StemFit%C3%82+Basic02+-+Stem+Cell+Culture+Media/custom%40sfb-500%4010%2E1038%2Fnprot%2E2016%2E170
Average 96 stars, based on 1 article reviews
stemfitâ basic02 - stem cell culture media - by Bioz Stars, 2026-10
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stemfit basic03  (Ajinomoto Althea)
95
Ajinomoto Althea stemfit basic03
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Stemfit Basic03, supplied by Ajinomoto Althea, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/StemFit+Basic03/custom%40sf031-001%4010%2E21203%2Frs%2E3%2Epex-2049
Average 95 stars, based on 1 article reviews
stemfit basic03 - by Bioz Stars, 2026-10
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stemfit ak03n  (Ajinomoto Althea)
96
Ajinomoto Althea stemfit ak03n
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Stemfit Ak03n, supplied by Ajinomoto Althea, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/StemFit+AK03N/custom%40sf010-002%4010%2E1038%2Fnprot%2E2016%2E170
Average 96 stars, based on 1 article reviews
stemfit ak03n - by Bioz Stars, 2026-10
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stemfit™ basic04ct  (ReproCELL)
90
ReproCELL stemfit™ basic04ct
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Stemfit™ Basic04ct, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/stemfit++basic04ct/bio_rxiv__2023__12__22__572808-123-18-20
Average 90 stars, based on 1 article reviews
stemfit™ basic04ct - by Bioz Stars, 2026-10
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feeder-less primate es cell medium reproff2  (ReproCELL)
90
ReproCELL feeder-less primate es cell medium reproff2
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Feeder Less Primate Es Cell Medium Reproff2, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/reproff+medium/pmc03374798-125-5-12
Average 90 stars, based on 1 article reviews
feeder-less primate es cell medium reproff2 - by Bioz Stars, 2026-10
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mtesr1  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc mtesr1
(A) Phase contrast light microscopic observation of cells during reprogramming and recloning. Images captured on a Pronectin F-coated dish prior to colony picking on days four, nine, 13, 17 and 24 (upper panels). Note that human ES cell-like colonies emerged within a cobblestone like morphology. (B) Efficiency of generating reprogrammed cells on various coating materials and the number of colonies characterized. All the experiments used 1×10 4 CD34 + CBCs, SeV TS vectors at 20 M.O.I. and ReproFF medium. Three independent experiments for Matrigel, Pronectin F and two independent experiments for fibronectin and laminin (Laminin-extracts), Pro nectin L we re performed. (C) Frequency of generating human ES cell-like colonies in various culture media. Five thousand CD34 + CBCs were infected with 20 M.O.I. of SeV carrying four factors and cultured in ReproFF, ReproFF2, <t>mTeSR1,</t> or E8 medium on Pronectin F-coated dishes to reprogram CD34+ CBCs. D: Endogenous gene expression of Klf4, c-Myc, Oct3/4, and Sox2 in feeder like-cells (lane 1) and first pick up of a human ES cell like-colony (lane 2).
Mtesr1, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/mtesr1+medium/pmc03374798-125-21-22
Average 90 stars, based on 1 article reviews
mtesr1 - by Bioz Stars, 2026-10
90/100 stars
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vectashield antifade mounting medium with dapi  (Vector Laboratories)
98
Vector Laboratories vectashield antifade mounting medium with dapi
(A) Phase contrast light microscopic observation of cells during reprogramming and recloning. Images captured on a Pronectin F-coated dish prior to colony picking on days four, nine, 13, 17 and 24 (upper panels). Note that human ES cell-like colonies emerged within a cobblestone like morphology. (B) Efficiency of generating reprogrammed cells on various coating materials and the number of colonies characterized. All the experiments used 1×10 4 CD34 + CBCs, SeV TS vectors at 20 M.O.I. and ReproFF medium. Three independent experiments for Matrigel, Pronectin F and two independent experiments for fibronectin and laminin (Laminin-extracts), Pro nectin L we re performed. (C) Frequency of generating human ES cell-like colonies in various culture media. Five thousand CD34 + CBCs were infected with 20 M.O.I. of SeV carrying four factors and cultured in ReproFF, ReproFF2, <t>mTeSR1,</t> or E8 medium on Pronectin F-coated dishes to reprogram CD34+ CBCs. D: Endogenous gene expression of Klf4, c-Myc, Oct3/4, and Sox2 in feeder like-cells (lane 1) and first pick up of a human ES cell like-colony (lane 2).
Vectashield Antifade Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/custom%40h-1200%4010%2E1038%2Fnprot%2E2016%2E170
Average 98 stars, based on 1 article reviews
vectashield antifade mounting medium with dapi - by Bioz Stars, 2026-10
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h9 human escs  (ReproCELL)
90
ReproCELL h9 human escs
(A) Phase contrast light microscopic observation of cells during reprogramming and recloning. Images captured on a Pronectin F-coated dish prior to colony picking on days four, nine, 13, 17 and 24 (upper panels). Note that human ES cell-like colonies emerged within a cobblestone like morphology. (B) Efficiency of generating reprogrammed cells on various coating materials and the number of colonies characterized. All the experiments used 1×10 4 CD34 + CBCs, SeV TS vectors at 20 M.O.I. and ReproFF medium. Three independent experiments for Matrigel, Pronectin F and two independent experiments for fibronectin and laminin (Laminin-extracts), Pro nectin L we re performed. (C) Frequency of generating human ES cell-like colonies in various culture media. Five thousand CD34 + CBCs were infected with 20 M.O.I. of SeV carrying four factors and cultured in ReproFF, ReproFF2, <t>mTeSR1,</t> or E8 medium on Pronectin F-coated dishes to reprogram CD34+ CBCs. D: Endogenous gene expression of Klf4, c-Myc, Oct3/4, and Sox2 in feeder like-cells (lane 1) and first pick up of a human ES cell like-colony (lane 2).
H9 Human Escs, supplied by ReproCELL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/h9+human+escs/pmc04747858-108-0-20
Average 90 stars, based on 1 article reviews
h9 human escs - by Bioz Stars, 2026-10
90/100 stars
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streptavidin biotin blocking kit  (Vector Laboratories)
99
Vector Laboratories streptavidin biotin blocking kit
(A) Phase contrast light microscopic observation of cells during reprogramming and recloning. Images captured on a Pronectin F-coated dish prior to colony picking on days four, nine, 13, 17 and 24 (upper panels). Note that human ES cell-like colonies emerged within a cobblestone like morphology. (B) Efficiency of generating reprogrammed cells on various coating materials and the number of colonies characterized. All the experiments used 1×10 4 CD34 + CBCs, SeV TS vectors at 20 M.O.I. and ReproFF medium. Three independent experiments for Matrigel, Pronectin F and two independent experiments for fibronectin and laminin (Laminin-extracts), Pro nectin L we re performed. (C) Frequency of generating human ES cell-like colonies in various culture media. Five thousand CD34 + CBCs were infected with 20 M.O.I. of SeV carrying four factors and cultured in ReproFF, ReproFF2, <t>mTeSR1,</t> or E8 medium on Pronectin F-coated dishes to reprogram CD34+ CBCs. D: Endogenous gene expression of Klf4, c-Myc, Oct3/4, and Sox2 in feeder like-cells (lane 1) and first pick up of a human ES cell like-colony (lane 2).
Streptavidin Biotin Blocking Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/Streptavidin/10__1038_slash_nprot__2016__170-195-18-21
Average 99 stars, based on 1 article reviews
streptavidin biotin blocking kit - by Bioz Stars, 2026-10
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lotus tetragonolobus lectin  (Vector Laboratories)
94
Vector Laboratories lotus tetragonolobus lectin
(A) Phase contrast light microscopic observation of cells during reprogramming and recloning. Images captured on a Pronectin F-coated dish prior to colony picking on days four, nine, 13, 17 and 24 (upper panels). Note that human ES cell-like colonies emerged within a cobblestone like morphology. (B) Efficiency of generating reprogrammed cells on various coating materials and the number of colonies characterized. All the experiments used 1×10 4 CD34 + CBCs, SeV TS vectors at 20 M.O.I. and ReproFF medium. Three independent experiments for Matrigel, Pronectin F and two independent experiments for fibronectin and laminin (Laminin-extracts), Pro nectin L we re performed. (C) Frequency of generating human ES cell-like colonies in various culture media. Five thousand CD34 + CBCs were infected with 20 M.O.I. of SeV carrying four factors and cultured in ReproFF, ReproFF2, <t>mTeSR1,</t> or E8 medium on Pronectin F-coated dishes to reprogram CD34+ CBCs. D: Endogenous gene expression of Klf4, c-Myc, Oct3/4, and Sox2 in feeder like-cells (lane 1) and first pick up of a human ES cell like-colony (lane 2).
Lotus Tetragonolobus Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/Unconjugated+Lotus+Tetragonolobus+Lectin+(LTL)/10__1038_slash_nprot__2016__170-195-101-105
Average 94 stars, based on 1 article reviews
lotus tetragonolobus lectin - by Bioz Stars, 2026-10
94/100 stars
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centrifuge  (Eppendorf AG)
99
Eppendorf AG centrifuge
(A) Phase contrast light microscopic observation of cells during reprogramming and recloning. Images captured on a Pronectin F-coated dish prior to colony picking on days four, nine, 13, 17 and 24 (upper panels). Note that human ES cell-like colonies emerged within a cobblestone like morphology. (B) Efficiency of generating reprogrammed cells on various coating materials and the number of colonies characterized. All the experiments used 1×10 4 CD34 + CBCs, SeV TS vectors at 20 M.O.I. and ReproFF medium. Three independent experiments for Matrigel, Pronectin F and two independent experiments for fibronectin and laminin (Laminin-extracts), Pro nectin L we re performed. (C) Frequency of generating human ES cell-like colonies in various culture media. Five thousand CD34 + CBCs were infected with 20 M.O.I. of SeV carrying four factors and cultured in ReproFF, ReproFF2, <t>mTeSR1,</t> or E8 medium on Pronectin F-coated dishes to reprogram CD34+ CBCs. D: Endogenous gene expression of Klf4, c-Myc, Oct3/4, and Sox2 in feeder like-cells (lane 1) and first pick up of a human ES cell like-colony (lane 2).
Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reproff2+reprocell+rchemd006/Eppendorf/pmc05278902-533-162-163
Average 99 stars, based on 1 article reviews
centrifuge - by Bioz Stars, 2026-10
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Image Search Results


Troubleshooting.

Journal: Nature protocols

Article Title: Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells

doi: 10.1038/nprot.2016.170

Figure Lengend Snippet: Troubleshooting.

Article Snippet: PBS (Life Technologies, 10010-049) ReproFF2 (ReproCELL, RCHEMD006) StemFit Basic (Ajinomoto, ASB01) Streptavidin/Biotin Blocking Kit (Vector Labs, SP-2002) Vectashield (Vector Labs, H-1200) Y-27632 dihydrochloride (TOCRIS, 1254)

Techniques: Concentration Assay, Microscopy

(A) Phase contrast light microscopic observation of cells during reprogramming and recloning. Images captured on a Pronectin F-coated dish prior to colony picking on days four, nine, 13, 17 and 24 (upper panels). Note that human ES cell-like colonies emerged within a cobblestone like morphology. (B) Efficiency of generating reprogrammed cells on various coating materials and the number of colonies characterized. All the experiments used 1×10 4 CD34 + CBCs, SeV TS vectors at 20 M.O.I. and ReproFF medium. Three independent experiments for Matrigel, Pronectin F and two independent experiments for fibronectin and laminin (Laminin-extracts), Pro nectin L we re performed. (C) Frequency of generating human ES cell-like colonies in various culture media. Five thousand CD34 + CBCs were infected with 20 M.O.I. of SeV carrying four factors and cultured in ReproFF, ReproFF2, mTeSR1, or E8 medium on Pronectin F-coated dishes to reprogram CD34+ CBCs. D: Endogenous gene expression of Klf4, c-Myc, Oct3/4, and Sox2 in feeder like-cells (lane 1) and first pick up of a human ES cell like-colony (lane 2).

Journal: PLoS ONE

Article Title: Generation of Virus-Free Induced Pluripotent Stem Cell Clones on a Synthetic Matrix via a Single Cell Subcloning in the Naïve State

doi: 10.1371/journal.pone.0038389

Figure Lengend Snippet: (A) Phase contrast light microscopic observation of cells during reprogramming and recloning. Images captured on a Pronectin F-coated dish prior to colony picking on days four, nine, 13, 17 and 24 (upper panels). Note that human ES cell-like colonies emerged within a cobblestone like morphology. (B) Efficiency of generating reprogrammed cells on various coating materials and the number of colonies characterized. All the experiments used 1×10 4 CD34 + CBCs, SeV TS vectors at 20 M.O.I. and ReproFF medium. Three independent experiments for Matrigel, Pronectin F and two independent experiments for fibronectin and laminin (Laminin-extracts), Pro nectin L we re performed. (C) Frequency of generating human ES cell-like colonies in various culture media. Five thousand CD34 + CBCs were infected with 20 M.O.I. of SeV carrying four factors and cultured in ReproFF, ReproFF2, mTeSR1, or E8 medium on Pronectin F-coated dishes to reprogram CD34+ CBCs. D: Endogenous gene expression of Klf4, c-Myc, Oct3/4, and Sox2 in feeder like-cells (lane 1) and first pick up of a human ES cell like-colony (lane 2).

Article Snippet: Reprogrammed cells were cultured in feeder-less primate ES cell medium Repro FF (ReproCELL, cat. No. RCHEMD004), ReproFF2 (ReproCELL, cat No. RCHEMD006), mTeSR1 (STEMCELL Technologies, catalog number 05850) or E8 (16) supplemented with five ng/mL bFGF (Peprotech) (total bFGF ten ng/mL) on Pronectin F-coated dishes.

Techniques: Infection, Cell Culture, Gene Expression